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Rat NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

  • Cat.No.:E-EL-R0662

  • Reactivity: Rat

To Purchase E-EL-R0662

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat NGAL in samples. No significant cross-reactivity or interference between Rat NGAL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat NGAL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NGAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NGAL. You can calculate the concentration of Rat NGAL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NGAL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NGAL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 196.68 439.76 1827.81 200.51 441.17 1920.91
Standard deviation 12.80 26.21 72.56 11.97 18.04 67.81
CV (%) 6.51 5.96 3.97 5.97 4.09 3.53

Recovery

The recovery of Rat NGAL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 95
EDTA plasma (n=8) 85-98 91
Cell culture media (n=8) 86-101 92

Linearity

Samples were spiked with high concentrations of Rat NGAL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsLCN2, Lipocalin 2, Oncogene 24p3, MSFI
Research AreaCancer, Cardiovascular, Metabolism, Immunology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Toxicology Letters (2020) IF: 3.569
    Effects of oral exposure to arsenite on arsenic metabolism and transport in rat kidney

    DOI: 10.1016/j.toxlet.2020.07.029

    PMID: 32736004

    Sample: Plasma,Urine
  2. Molecular and Cellular Biochemistry (2019) IF: 2.795
    The protective effect of cannabinoid type 2 receptor activation on renal ischemia–reperfusion injury

    DOI: 10.1007/s11010-019-03616-6

    PMID: 31446615

    Sample: Serum
  3. Human & Experimental Toxicology (2020) IF: 2.067
    Protective effect of febuxostat in sepsis-induced liver and kidney injuries after cecal ligation and puncture with the impact of xanthine oxidase, interleukin 1 β, and c-Jun N-terminal kinases

    DOI: 10.1177/0960327120905957

    PMID: 32054342

    Sample: Serum,Tissue homogenate
  4. European Journal of Trauma and Emergency Surgery (2019) IF: 2.139
    Beneficial effects of bardoxolone methyl, an Nrf2 activator, on crush-related acute kidney injury in rats

    DOI: 10.1007/s00068-019-01216-z

    PMID: 31471671

    Sample: Tissue homogenate
  5. journal of surgical research (2019) IF: 2.051
    Effect of Hydrogen Sulfide on Kidney Injury in Rat Model of Crush Syndrome

    DOI: 10.1016/j.jss.2018.10.033

    PMID: 30691831

    Sample: Tissue homogenate
  6. Journal of cellular biochemistry (2019) IF: 2.959
    Protective effect of N-(p-amylcinnamoyl) anthranilic acid, phospholipase A2 enzyme inhibitor, and transient receptor potential melastatin-2 channel blocker against renal ischemia–reperfusion injury

    DOI: 10.1002/jcb.27664

    PMID: 30259992

    Sample: Serum
  7. Medical science monitor: international medical journal of experimental and clinical research (2019) IF: 1.918
    miR-30c-5p Reduces Renal Ischemia-Reperfusion Involving Macrophage

    DOI: 10.12659/MSM.914579

    PMID: 31185006

    Sample: Serum
  8. Biological and Pharmaceutical Bulletin (2018) IF: 1.54
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    Sample: Plasma
  9. International urology and nephrology (2019) IF: 1.692
    Effects of hydrogen sulfide on acetaminophen-induced acute renal toxicity in rats

    DOI: 10.1007/s11255-018-2053-0

    PMID: 30604234

    Sample: Tissue homogenate
  10. Plant Archives (2019)
    EVALUATION OF EFFECTIVENESS OF MYRRH GUM EXTRACT ON SOME BIOCHEMICAL AND HISTOLOGICAL PARAMETERS IN MALE RATS INDUCED CHRONIC RENAL FAILURE (CRF)
    Sample: Serum
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