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Current Position:Home>>Kits>>ELISA Kits>> Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Citations(144)Uniprot : P16599

  • Cat.No.:E-EL-R0019

  • Reactivity: Rat

To Purchase E-EL-R0019

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 78.13-5000 pg/mL
Sensitivity 46.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat TNF-α in samples. No significant cross-reactivity or interference between Rat TNF-α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat TNF-α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNF-α. You can calculate the concentration of Rat TNF-α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TNF-α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 249.60 624.50 2364.40 243.60 673.40 2259.40
Standard deviation 15.50 34.30 85.10 12.40 39.70 74.60
CV (%) 6.21 5.49 3.60 5.09 5.90 3.30

Recovery

The recovery of Rat TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-100 95
EDTA plasma (n=8) 93-108 100
Cell culture media (n=8) 88-102 95

Linearity

Samples were spiked with high concentrations of Rat TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P16599
SynonymsDIF, TNF-alpha, TNFA, TNFSF2
Research AreaCancer,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

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  2. British journal of pharmacology (2018) IF: 6.81
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    DOI: 10.1111/bph.14500

    PMID: 30221753

    		 Sample: Serum
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    DOI: 10.1155/2020/1720961

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  4. International Journal of Biological Macromolecules (2020) IF: 5.162
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  5. Journal of Neuroinflammation (2020) IF: 5.793
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    DOI: 10.1186/s12974-020-01891-5

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    		 Sample: Tissue homogenate
  6. Journal of Cellular Physiology (2020) IF: 5.546
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    DOI: 10.1002/jcp.29453

    PMID: 31960959

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  7. Materials Science and Engineering: C (2020) IF: 5.88
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    DOI: 10.1007/s12975-019-00715-w

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    		 Sample: Plasma
  9. Chemosphere (2019) IF: 5.778
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    DOI: 10.1016/j.chemosphere.2019.124501

    PMID: 31398612

    		 Sample: Serum
  10. Aging (Albany NY) (2018) IF: 5.515
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    DOI: 10.18632/aging.101627

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    		 Sample: Tissue homogenate
  11. development (2019) IF: 5.413
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    DOI: 99999999

    PMID: 99999999

    		 Sample: Serum
  12. Toxicology (2020) IF: 4.099
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    DOI: 10.1016/j.tox.2020.152556

    PMID: 32758512

    		 Sample: Serum,Tissue homogenate
  13. European Journal of Pharmaceutics and Biopharmaceutics (2020) IF: 4.604
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    DOI: 10.1016/j.ejpb.2020.07.009

    PMID: 32681961

    		 Sample: Serum
  14. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110075

    PMID: 32179202

    		 Sample: Serum,Tissue homogenate
  15. Biochemical Pharmacology (2020) IF: 4.96
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    DOI: 10.1016/j.bcp.2019.113681

    PMID: 31669235

    		 Sample: Tissue homogenate
  16. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110400

    PMID: 32570115

    		 Sample: Serum
  17. Ecotoxicology and Environmental Safety (2020) IF: 4.872
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    DOI: 10.1016/j.ecoenv.2019.110065

    PMID: 31869719

    		 Sample: Tissue homogenate
  18. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110471

    PMID: 99999999

    		 Sample: Serum
  19. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110273

    PMID: 32460188

    		 Sample: Cell culture supernatant
  20. Nutrients (2020) IF: 4.546
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    DOI: 10.3390/nu12051426

    PMID: 32423176

    		 Sample: Serum
  21. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Berberine regulates fecal metabolites to ameliorate 5-fluorouracil induced intestinal mucositis through modulating gut microbiota

    DOI: 10.1016/j.biopha.2020.109829

    PMID: 31958765

    		 Sample: Tissue homogenate
  22. Frontiers in Pharmacology (2020) IF: 4.225
    Sodium Tanshinone II Sulfonate A Ameliorates Hypoxia-Induced Pulmonary Hypertension

    DOI: 10.3389/fphar.2020.00687

    PMID: 32508639

    		 Sample: Tissue homogenate
  23. International Journal of Biological Sciences (2019) IF: 4.858
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    DOI: 10.7150/ijbs.38315

    PMID: 31853224

    		 Sample: Serum,Bronchoalveolar Lavage Fluid,Tissue homogena
  24. Journal of cellular biochemistry (2019) IF: 4.237
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    DOI: 10.1002/jcb.28976

    PMID: 31099131

    		 Sample: Tissue homogenate
  25. Neurobiology of aging (2019) IF: 4.454
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    DOI: 10.1016/j.neurobiolaging.2018.11.023

    PMID: 30594047

    		 Sample: Tissue homogenate,Cell culture medium
  26. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2019) IF: 4.328
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    DOI: 10.1016/j.bbadis.2019.02.020

    PMID: 30826466

    		 Sample: Serum
  27. Scientific reports (2019) IF: 4.122
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    DOI: 10.1038/s41598-019-40969-w

    PMID: 30858526

    		 Sample: Serum
  28. International Immunopharmacology (2020) IF: 3.943
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    DOI: 10.1016/j.intimp.2020.106771

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    		 Sample: Serum
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    DOI: 10.1016/j.imlet.2020.06.010

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    		 Sample: Serum
  30. Environmental Toxicology and Pharmacology (2020) IF: 3.292
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    		 Sample: Serum
  32. Mycotoxin Research (2020) IF: 3.164
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    DOI: 10.1007/s12550-020-00408-6

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    		 Sample: Serum
  33. European review for medical and pharmacological sciences (2020) IF: 3.024
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    DOI: 10.26355/eurrev_202006_21528

    PMID: 99999999

    		 Sample: Serum
  34. Environmental Toxicology (2020) IF: 3.118
    Kolaviron ameliorates hepatic and renal dysfunction associated with multiwalled carbon nanotubes in rats

    DOI: 10.1002/tox.23011

    PMID: 32856799

    		 Sample: Serum
  35. Environmental Science and Pollution Research (2020) IF: 3.056
    Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats

    DOI: 10.1007/s11356-020-10324-y

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    		 Sample: Serum,Tissue homogenate
  36. Environmental Science and Pollution Research (2020) IF: 3.056
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    DOI: 10.1007/s11356-020-10042-5

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    		 Sample: Serum
  37. Cell Cycle (2020) IF: 3.699
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    DOI: 10.1080/15384101.2020.1814025

    PMID: 32897801

    		 Sample: Cell culture supernatant
  38. Psychopharmacology (2020) IF: 3.13
    Kolaviron via anti-inflammatory and redox regulatory mechanisms abates multi-walled carbon nanotubes-induced neurobehavioral deficits in rats

    DOI: 10.1007/s00213-019-05432-8

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    		 Sample: Tissue homogenate
  39. Journal of Ethnopharmacology (2020) IF: 3.69
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    DOI: 10.1016/j.jep.2020.113039

    PMID: 32497675

    		 Sample: Serum,Cell culture supernatant
  40. Mediators of Inflammation (2020) IF: 3.758
    Paeonol Attenuates Methotrexate-Induced Cardiac Toxicity in Rats by Inhibiting Oxidative Stress and Suppressing TLR4-Induced NF- κ B Inflammatory Pathway

    DOI: 10.1155/2020/8641026

    PMID: 32104151

    		 Sample: Soil,Water,Oil,Plant?Tissue,Plant?Fluid,Antibody,F
  41. Journal of Biochemical and Molecular Toxicology (2020) IF: 3.606
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    DOI: 10.1002/jbt.22450

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    		 Sample: Tissue homogenate
  42. Inflammopharmacology (2020) IF: 3.238
    Moringa rivae leaf extracts attenuate Complete Freund's adjuvant-induced arthritis in Wistar rats via modulation of inflammatory and oxidative stress biomarkers

    DOI: 10.1007/s10787-019-00596-3

    PMID: 31037575

    		 Sample: Serum
  43. Environmental Toxicology (2020) IF: 3.118
    Cadmium and nickel co-exposure exacerbates genotoxicity and not oxido-inflammatory stress in liver and kidney of rats: Protective role of omega-3 fatty acid

    DOI: 10.1002/tox.22860

    PMID: 31639282

    		 Sample: Tissue homogenate
  44. International Immunopharmacology (2020) IF: 3.943
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    DOI: 10.1016/j.intimp.2020.106582

    PMID: 32438076

    		 Sample: Plasma
  45. Obesity (2020) IF: 3.742
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    DOI: 10.1002/oby.22666

    PMID: 31773909

    		 Sample: Cell culture supernatant
  46. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    Overexpression of HOXA-AS2 inhibits inflammation and apoptosis in podocytes via sponging miR-A-302b-3p to upregulate TIMP3

    DOI: 10.26355/eurrev_202005_21187

    PMID: 32432759

    		 Sample: Serum
  47. Inflammation (2020) IF: 3.212
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    DOI: 10.1007/s10753-019-01123-w

    PMID: 31705353

    		 Sample: Serum
  48. Journal of Biochemical and Molecular Toxicology (2020) IF: 3.606
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    DOI: 10.1002/jbt.22427

    PMID: 31777137

    		 Sample: Serum,Tissue homogenate
  49. International Immunopharmacology (2020) IF: 3.943
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    DOI: 10.1016/j.intimp.2019.105978

    PMID: 31767546

    		 Sample: Tissue homogenate
  50. NeuroToxicology (2020) IF: 3.105
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    DOI: 10.1016/j.neuro.2019.10.009

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    		 Sample: Tissue homogenate
  51. Biology of Reproduction (2019) IF: 3.322
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    DOI: 10.1093/biolre/ioz133

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    		 Sample: Tissue homogenate
  52. European Journal of Pharmacology (2019) IF: 3.263
    Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

    DOI: 10.1016/j.ejphar.2019.172786

    PMID: 31712060

    		 Sample: Serum
  53. Life Sciences (2019) IF: 3.647
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    DOI: 10.1016/j.lfs.2019.117045

    PMID: 31730866

    		 Sample: Serum
  54. Neuroscience (2018) IF: 3.056
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    DOI: 10.1016/j.neuroscience.2018.09.041

    PMID: 30300703

    		 Sample: Serum
  55. Inflammopharmacology (2018) IF: 3.304
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    DOI: 10.1007/s10787-018-0522-9

    PMID: 30094758

    		 Sample: Tissue homogenate
  56. Apoptosis (2018) IF: 3.967
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    DOI: 10.1007/s10495-018-1474-7

    PMID: 30167920

    		 Sample: Serum
  57. Biomedicine & Pharmacotherapy (2019) IF: 3.457
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    DOI: 10.1016/j.biopha.2018.10.060

    PMID: 30396096

    		 Sample: Serum
  58. International Journal of Neuropsychopharmacology (2018) IF: 3.981
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    DOI: 10.1093/ijnp/pyy090

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    		 Sample: Plasma,Tissue homogenate
  59. Frontiers in pharmacology (2018) IF: 3.831
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    DOI: 10.3389/fphar.2018.01432

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    		 Sample: Serum,Cell culture supernatant
  60. Molecules (2019) IF: 3.098
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    		 Sample: Serum
  61. Journal of cellular physiology (2019) IF: 3.923
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    DOI: 10.1002/jcp.28126

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    		 Sample: Serum
  62. Molecules (2019) IF: 3.098
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    DOI: 10.3390/molecules24020340

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    		 Sample: Plasma,Tissue homogenate
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    DOI: 10.1016/j.jtemb.2019.01.014

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    		 Sample: Tissue homogenate
  64. Ecotoxicology and environmental safety (2019) IF: 3.974
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    DOI: 10.1016/j.ecoenv.2018.10.064

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    		 Sample: Tissue homogenate
  65. Life sciences (2019) IF: 3.234
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    DOI: 10.1016/j.lfs.2019.02.048

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    		 Sample: Serum,Tissue homogenate
  66. Environmental Toxicology and Pharmacology (2020) IF: 3.292
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    DOI: 10.1016/j.etap.2020.103387

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    		 Sample: Serum
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    DOI: 10.3389/fnins.2020.00659

    PMID: 99999999

    		 Sample: Tissue homogenate
  68. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2020) IF: 2.842
    Activation of Nrf2 Signaling by Apelin Attenuates Renal Ischemia Reperfusion Injury in Diabetic Rats

    DOI: 10.2147/DMSO.S246743

    PMID: 32606875

    		 Sample: Serum
  69. Toxin Reviews (2020) IF: 2.851
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    DOI: 10.1080/15569543.2020.1790604

    PMID: 99999999

    		 Sample: Tissue homogenate
  70. Toxicology Research (2020) IF: 2.283
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    DOI: 10.1093/toxres/tfaa060/5898655

    PMID: 99999999

    		 Sample: Tissue homogenate
  71. Therapeutic Advances in Endocrinology and Metabolism (2020) IF: 2.173
    Plant-derived oleanolic acid ameliorates markers of subclinical inflammation and innate immunity activation in diet-induced pre-diabetic rats

    DOI: 10.1177/2042018820935771

    PMID: 32782775

    		 Sample: Plasma
  72. Molecular Medicine Reports (2020) IF: 2.1
    Pretreatment with the ALDH2 activator Alda-1 protects rat livers from ischemia/reperfusion injury by inducing autophagy

    DOI: 10.3892/mmr.2020.11312

    PMID: 32705206

    		 Sample: Serum
  73. Journal of the Science of Food and Agriculture (2020) IF: 2.614
    Tiliacora triandra extract and its major constituent attenuates diabetic kidney and testicular impairment by modulating redox imbalance and pro-inflammatory responses in rats

    DOI: 10.1002/jsfa.10779

    PMID: 32875596

    		 Sample: Tissue homogenate
  74. Immunological Investigations (2020) IF: 2.511
    Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase-1/IL-1β Signaling Pathway Inhibition

    DOI: 10.1080/08820139.2020.1791178

    PMID: 32674625

    		 Sample: Cell culture supernatant
  75. Human & Experimental Toxicology (2020) IF: 2.067
    Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats

    DOI: 10.1177/0960327120960775

    PMID: 32959702

    		 Sample: Tissue homogenate
  76. Fundamental & Clinical Pharmacology (2020) IF: 2.754
    Ameliorative effect of Ozagrel, a thromboxane A2 synthase inhibitor, in hyperhomocysteinemia-induced experimental vascular cognitive impairment and dementia

    DOI: 10.1111/fcp.12610

    PMID: 33020931

    		 Sample: Tissue homogenate
  77. Experimental Physiology (2020) IF: 2.431
    Combination of quercetin and exercise training attenuates depression in rats with 1,2-dimethylhydrazine-induced colorectal cancer: Possible involvement of inflammation and BDNF signalling

    DOI: 10.1113/EP088605

    PMID: 32681548

    		 Sample: Serum,Tissue homogenate
  78. Molecular Medicine Reports (2020) IF: 2.1
    Placenta-derived mesenchymal stem cells ameliorate lipopolysaccharide-induced inflammation in RAW264. 7 cells and acute lung injury in rats

    DOI: 10.3892/mmr.2020.11231

    PMID: 32626979

    		 Sample: Cell culture supernatant
  79. Clinical Rheumatology (2020) IF: 2.394
    Investigation of the efficacy of daidzein in experimental knee osteoarthritis-induced with monosodium iodoacetate in rats

    DOI: 10.1007/s10067-020-04958-z

    PMID: 32103372

    		 Sample: Serum
  80. European Journal of Trauma and Emergency Surgery (2019) IF: 2.139
    Beneficial effects of bardoxolone methyl, an Nrf2 activator, on crush-related acute kidney injury in rats

    DOI: 10.1007/s00068-019-01216-z

    PMID: 31471671

    		 Sample: Tissue homogenate
  81. Journal of Ovarian Research (2020) IF: 2.989
    The effect of glutamine on Dehydroepiandrosterone-induced polycystic ovary syndrome rats

    DOI: 10.1186/s13048-020-00650-7

    PMID: 32386521

    		 Sample: Serum
  82. Digestive Diseases and Sciences (2020) IF: 2.751
    Apocynin Attenuates Acute Kidney Injury and Inflammation in Rats with Acute Hypertriglyceridemic Pancreatitis

    DOI: 10.1007/s10620-019-05892-0

    PMID: 31617131

    		 Sample: Serum
  83. PloS one (2020) IF: 2.74
    Associations of inflammatory markers with impaired left ventricular diastolic and systolic function in collagen-induced arthritis

    DOI: 10.1371/journal.pone.0230657

    PMID: 32208438

    		 Sample: Serum
  84. Future Microbiology (2020) IF: 2.907
    The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model

    DOI: 10.2217/fmb-2020-0005

    PMID: 32250184

    		 Sample: Serum
  85. Naunyn-Schmiedeberg's archives of pharmacology (2020) IF: 2.05
    Protective effect of oleuropein on ketamine-induced cardiotoxicity in rats

    DOI: 10.1007/s00210-020-01870

    PMID: 99999999

    		 Sample: Serum
  86. Cancer chemotherapy and pharmacology (2020) IF: 2.967
    The gut microbiota attenuates muscle wasting by regulating energy metabolism in chemotherapy-induced malnutrition rats

    DOI: 10.1007/s00280-020-04060-w

    PMID: 32415349

    		 Sample: Serum,Tissue homogenate,Feces
  87. Saudi Pharmaceutical Journal (2020) IF: 2.879
    Possible combined effect of perindopril and Azilsartan in an experimental model of dementia in rats

    DOI: 10.1016/j.jsps.2020.03.009

    PMID: 32435138

    		 Sample: Tissue homogenate
  88. Experimental and Clinical Endocrinology & Diabetes (2019) IF: 2.058
    Hyperuricemia Inhibition Protects SD Rats Against Fructose-Induced Obesity Hypertension Via Modulation of Inflammation and Renin-Angiotensin System in Adipose Tissue

    DOI: 10.1055/a-1023-6710

    PMID: 31683330

    		 Sample: Serum
  89. Archives of Physiology and Biochemistry (2019) IF: 2.575
    Evaluating the effects of Juglans regia L. extract on hyperglycaemia and insulin sensitivity in experimental type 2 diabetes in rat

    DOI: 10.1080/13813455.2019.1668018

    PMID: 99999999

    		 Sample: Plasma
  90. Wound Repair and Regeneration (2018) IF: 2.952
    Therapeutic efficacy of early photobiomodulation therapy on the zones of stasis in burns: An experimental rat model study

    DOI: 10.1111/wrr.12661

    PMID: 30118166

    		 Sample: Tissue homogenate
  91. Canadian journal of physiology and pharmacology (2018) IF: 2.21
    Histopathological and biochemical changes in the development of nonalcoholic fatty liver disease induced by high-sucrose diet at different times

    DOI: 10.1139/cjpp-2018-0353

    PMID: 30388378

    		 Sample: Serum
  92. Bioscience reports (2018) IF: 2.899
    Shenqi Fuzheng Injection impairs bile duct ligation-induced cholestatic liver injury in vivo

    DOI: 10.1042/BSR20180787

    PMID: 30610157

    		 Sample: Tissue homogenate
  93. American Journal of Reproductive Immunology (2019) IF: 2.745
    Nisin Reduces Uterine Inflammation in Rats by Modulating Concentrations of Pro- And Anti-Inflammatory Cytokines

    DOI: 10.1111/aji.13096

    PMID: 30681748

    		 Sample: Serum
  94. Biochemical and biophysical research communications (2018) IF: 2.559
    Serum thyroid hormones levels are significantly decreased in pregnant rats with acute pancreatitis

    DOI: 10.1016/j.bbrc.2018.09.180

    PMID: 30286956

    		 Sample: Serum
  95. Canadian journal of physiology and pharmacology (2018) IF: 2.21
    The pectin-insulin patch application prevents the onset of peripheral neuropathy-like symptoms in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2018-0415

    PMID: 30326192

    		 Sample: Plasma
  96. Journal of cellular biochemistry (2019) IF: 2.959
    Exosomes derived from miR-146a-modified adipose- derived stem cells attenuate acute myocardial infarction- induced myocardial damage via downregulation of early growth response factor 1

    DOI: 10.1002/jcb.27731

    PMID: 30362610

    		 Sample: Serum,Cell culture supernatant
  97. Journal of cellular biochemistry (2019) IF: 2.959
    MAPKs and NF-κB-mediated acrylamide-induced neuropathy in rat striatum and human neuroblastoma cells SY5Y

    DOI: 10.1002/jcb.27671

    PMID: 30368882

    		 Sample: Tissue homogenate
  98. Autoimmunity (2019) IF: 2.648
    The changes in immune cell concentration during the progression of pre-diabetes to type 2 diabetes in a high-fat high-carbohydrate diet-induced pre-diabetic rat model

    DOI: 10.1080/08916934.2019.1575820

    PMID: 30776930

    		 Sample: Plasma
  99. ACS omega (2020) IF: 2.87
    Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies

    DOI: 10.1021/acsomega.0c01670

    PMID: 32803042

    		 Sample: Serum
  100. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    		 Sample: Plasma,Tissue homogenate
  101. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology (2020) IF: 1.805
    The hepato-protective effect of eupatilin on an alcoholic liver disease model of rats

    DOI: 10.4196/kjpp.2020.24.5.385

    PMID: 32830145

    		 Sample: Tissue homogenate
  102. Medicine (2019) IF: 1.552
    Investigation of biochemical and histopathological effects of tarantula cubensis D6 on lungtissue in cecal ligation and puncture-inducedpolymicrobial sepsis model in rats

    DOI: 10.5455/medscience.2019.08.9045

    PMID: 99999999

    		 Sample: Tissue homogenate
  103. International urology and nephrology (2020) IF: 1.843
    Vinpocetine reduces cisplatin-induced acute kidney injury through inhibition of NF-κB pathway and activation of Nrf2/ARE pathway in rats

    DOI: 10.1007/s11255-020-02485-z

    PMID: 32418008

    		 Sample: Serum,Urine,Tissue homogenate
  104. Asian Journal of Surgery (2020) IF: 1.838
    Potent therapeutic effects of ruscogenin on gastric ulcer established by acetic acid

    DOI: 10.1016/j.asjsur.2019.07.001

    PMID: 31345657

    		 Sample: Tissue homogenate
  105. Andrologia (2020) IF: 1.951
    Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM-1 and VEGF-A

    DOI: 10.1111/and.13472

    PMID: 31773790

    		 Sample: Serum
  106. in vivo (2019) IF: 1.541
    Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats

    DOI: 10.21873/invivo.11624

    PMID: 31471392

    		 Sample: Bronchoalveolar Lavage Fluid
  107. Annals of Surgical Treatment and Research (2019) IF: 1.367
    Examination of Protective and Therapeutic Effects of Ruscogenin on Cerulein-Induced Experimental Acute Pancreatitis in Rats

    DOI: 10.4174/astr.2019.97.6.271

    PMID: 31824881

    		 Sample: Serum,Tissue homogenate
  108. Biological and Pharmaceutical Bulletin (2018) IF: 1.54
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    		 Sample: Plasma
  109. Experimental and therapeutic medicine (2018) IF: 1.41
    Protective effect of dexpanthenol against cisplatin-induced hepatotoxicity

    DOI: 10.3892/etm.2018.6683

    PMID: 30402149

    		 Sample: Tissue homogenate
  110. Journal of Food Biochemistry (2019) IF: 1.552
    Epicatechin and scopoletin rich Morinda citrifolia (Noni) leaf extract supplementation, mitigated Osteoarthritis via anti-inflammatory, anti-oxidative, and anti-protease pathways

    DOI: 10.1111/jfbc.12755

    PMID: 31353568

    		 Sample: Serum
  111. Journal of biochemical and molecular toxicology (2019) IF: 1.837
    Fluoride and diethylnitrosamine coexposure enhances oxido-inflammatory responses and caspase-3 activation in liver and kidney of adult rats

    DOI: 10.1002/jbt.22327

    PMID: 30920066

    		 Sample: Serum
  112. Renal failure (2019) IF: 1.44
    The aqueous extract of aged black garlic ameliorates colistin-induced acute kidney injury in rats

    DOI: 10.1080/0886022X.2018.1561375

    PMID: 30714460

    		 Sample: Tissue homogenate
  113. Journal of Inflammation Research (2020)
    Pharmacological Inhibition of Galectin-3 Ameliorates Diabetes-Associated Cognitive Impairment, Oxidative Stress and Neuroinflammation in vivo and in vitro

    DOI: 10.2147/JIR.S273858

    PMID: 32982368

    		 Sample: Cell culture supernatant,Tissue homogenate
  114. Naunyn-Schmiedeberg's Archives of Pharmacology (2020)
    The protective effect of fenofibrate, triptorelin, and their combination against premature ovarian failure in rats

    DOI: 10.1007/s00210-020-01975-2

    PMID: 32924068

    		 Sample: Serum
  115. Naunyn-Schmiedeberg's archives of pharmacology (2020)
    Protective effect of oleuropein on ketamine-induced cardiotoxicity in rats

    DOI: 10.1007/s00210-020-01870-w

    PMID: 32383030

    		 Sample: Tissue homogenate
  116. Journal of Family and Reproductive Health (2020)
    Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats

    DOI: 10.1177/1535370220931038

    PMID: 32493122

    		 Sample: Tissue homogenate
  117. Advances in Traditional Medicine (2020)
    Anti-inflammatory activity of Xanthium indicum on carrageenan-induced paw edema in rats

    DOI: 10.1007/s13596-020-00502-1

    PMID: 99999999

    		 Sample: Tissue homogenate
  118. Medicinal Plant (2017)
    Protection of Dicliptera chinensis Polysaccharide Granule on Acute Liver Injury of Rat Caused by D-galactosamine

    DOI: 99999999

    PMID: 99999999

    		 Sample: Serum,Tissue Slice
  119. Annals of Tropical Medicine & Public Health (2019)
    Protective effect of Melatonin, Rosuvastatin and their combination against Amikacin induced nephrotoxicity in rats

    DOI: 10.36295/ASRO.2019.220513

    PMID: 99999999

    		 Sample: Serum
  120. Indonesian Journal of Environmental Management and Sustainability (2020)
    Effectiveness of Spirulina platensis Extract on Wound Area and TNF-a Levels on Blood: Experimental Studies In Wistar Rats Made Artificially by Vulnus Scissum and Infected by Staphylococcus aureus

    DOI: 10.26554/ijems.2020.4.2.55-58

    PMID: 99999999

    		 Sample: Serum
  121. Asian Journal of Research in Biochemistry (2019)
    Effect of Oral Intake of Sodium Benzoate on Serum Cholesterol and Proinflammatory Cytokine (Tumor Necrosis Factor Alpha [TNF-α] and Interleukin-6 [IL-6]) Levels in the Heart Tissue of Wistar Rats

    DOI: 10.9734/ajrb/2019/v5i230086

    PMID: 99999999

    		 Sample: Serum,Tissue homogenate
  122. Plant Archives (2020)
    PROTECTIVE EFFECT OF MANDARIN (CITRUS RETICULATA) PEEL EXTRACT ON POTASSIUM DICHROMATE INDUCED HEPATOTOXICITY AND NEPHROTOXICITY IN RATS

    DOI: 99999999

    PMID: 99999999

    		 Sample: Tissue homogenate
  123. Eastern Anatolian Journal of Science (2020)
    The Possible Beneficial Effects of Lazaroid U-74389G on Ovarian Injury

    DOI: 99999999

    PMID: 99999999

    		 Sample: Tissue homogenate
  124. New Trends in Medicine Sciences (2020)
    Role of Hyperoside on Ovarian Tissue Damage Created by Ovarian Torsion Detorsion

    DOI: 99999999

    PMID: 99999999

    		 Sample: Tissue homogenate
  125. The Egyptian Journal of Forensic Sciences and Applied Toxicology (2020)
    ANTI-INFLAMMATORY AND ANTIOXIDANT PROPERTIES OF ECBALLIUM ELATERIUM FRUIT JUICE AGAINST CYCLOPHPSPHAMIDE INDUCED HEPATOTOXICITY IN RATS

    DOI: 99999999

    PMID: 99999999

    		 Sample: Plasma
  126. GSC Biological and Pharmaceutical Sciences (2019)
    Protective effects of naringin in indomethacin-induced gastric ulcer in rats

    DOI: 10.30574/gscbps.2019.8.2.0132

    PMID: 99999999

    		 Sample: Tissue homogenate
  127. Preprint (2020)
    Therapeutic effects of different treatment periods against ischemic stroke and toxicology study of Angong Niuhuang Pill in rats

    DOI: 10.21203/rs.2.21991/v2

    PMID: 99999999

    		 Sample: Serum,Tissue homogenate
  128. Journal of Complementary and Alternative Medical Research (2019)
    Hydroethanolic Extract of Buchholzia coriacea Seeds Alleviates LPS Induced Liver Injury in Rat via Antioxidant and Anti-inflammatory Actions

    DOI: 10.9734/jocamr/2019/v8i230116

    PMID: 99999999

    		 Sample: Serum
  129. International Journal of Clinical and Experimental Pathology (2020) IF: 0.252
    Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway

    DOI: 99999999

    PMID: 32509067

    		 Sample: Serum
  130. The American Journal of Chinese Medicine (2020)
    Corydalis saxicola Alkaloids Attenuate Cisplatin-Induced Neuropathic Pain by Reducing Loss of IENF and Blocking TRPV1 Activation

    DOI: 10.1142/S0192415X20500214

    PMID: 32138533

    		 Sample: Serum
  131. Acta Cirurgica Brasileira (2020) IF: 0.974
    TNF-alpha inhibitor Adal?mumab attenuates endotoxin induced cardiac damage in rats

    DOI: 10.1590/s0102-865020200020000002

    PMID: 32267288

    		 Sample: Serum
  132. Acta Cirurgica Brasileira (2019) IF: 0.974
    Effect of sevoflurane pretreatment in relieving liver ischemia/reperfusion-induced pulmonary and hepatic injury

    DOI: 10.1590/s0102-865020190080000005

    PMID: 31618405

    		 Sample: Serum
  133. Nigerian Journal of Physiological Sciences (2018)
    Effect of caffeine on Serum tumour necrosis factor alpha and lactate dehydrogenase in Wistar rats exposed to cerebral ischaemia-reperfusion injury

    DOI: 99999999

    PMID: 30091726

    		 Sample: Serum
  134. Pathology-Research and Practice (2018)
    miR-93-3p alleviates lipopolysaccharide-induced inflammation and apoptosis in H9c2 cardiomyocytes by inhibiting toll-like receptor 4

    DOI: 10.1016/j.prp.2018.08.024

    PMID: 30195636

    		 Sample: Cell culture supernatant
  135. Mustansiriya Medical Journal (2018)
    Evaluation of the effects of TAK-242 and GIT-27 on methotrexate-induced liver injury

    DOI: 10.4103/MJ.MJ_15_18

    PMID: 99999999

    		 Sample: Serum
  136. Annals of RSCB (2018)
    The antioxidants are not enough. Malus sylvestris (L.) Mill. extract enhances the carbon tetrachloride liver toxicity in albino rats.

    DOI: 10.ANN/RSCB-2018-00018:RSCB

    PMID: 99999999

    		 Sample: Plasma
  137. Journal of Pharmaceutical Sciences and Research (2018)
    Role of hydroxytyrosol in ameliorating effects of high fat diet on male rats CNS

    DOI: 99999999

    PMID: 99999999

    		 Sample: Serum
  138. Jurnal Bio Komplementer Medicine (2019)
    PENGARUH KOMBINASI EKSTRAK AIR DAUN JATI BELANDA, KEMUNING, MURBEI, DAN RIMPANG BANGLE TERHADAP KADAR TNF-α Serum TIKUS DENGAN DIET TINGGI LEMAK

    DOI: 99999999

    PMID: 99999999

    		 Sample: Serum
  139. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2019)
    Cardioprotective effects of a ruthenium (II) Schiff base complex in diet-induced prediabetic rats.

    DOI: 10.2147/DMSO.S183811

    PMID: 30858714

    		 Sample: Tissue homogenate,Plasma
  140. Toxicology Reports (2019)
    Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos

    DOI: 10.1016/j.toxrep.2019.02.007

    PMID: 30859069

    		 Sample: Tissue homogenate
  141. Pathophysiology (2019)
    Pathophysiology of iron overload-induced renal injury and dysfunction: Roles of renal oxidative stress and systemic inflammatory mediators

    DOI: 10.1016/j.pathophys.2019.03.002

    PMID: 30910397

    		 Sample: Serum
  142. Clinical Cancer Drugs (2019)
    Coenzyme Q10 Attenuates Cisplatin-induced Nephrotoxicity Through Counteracting Oxidative Stress and Inflammation

    DOI: 10.2174/2212697X06666190701113043

    PMID: 99999999

    		 Sample: Serum,Tissue homogenate
  143. Journal of Diabetes and Islet Biology (2020)
    Assessment of the Effects of Crude Methanolic Extracts (Leaf and Twig) of Loranthus micranthus on Streptozotocin Induced Diabetic Rats

    DOI: 10.31579/ 2641-8975/015

    PMID: 99999999

    		 Sample: Plasma
  144. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.166
    Effects of methylene blue on the nitric oxide-soluble guanylate cyclase-cyclic guanylyl monophosphate pathway and cytokine levels in rats with sepsis

    DOI: 99999999

    PMID: 99999999

    		 Sample: Serum,Tissue homogenate
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