T(Testosterone) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
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    >
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-0072

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 0.10~20 ng/mL

      Sensitivity: 0.10 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual
      可替代产品推荐: T(Testosterone) ELISA Kit

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma and other biological fluids.


      Test principle

      This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Goat Anti-Rabbit IgG, make solid-phase secondary antibody. And then add samples, horseradish peroxidase-labeled Testosterone and anti- Testosterone antibody, so as to form a coated secondary antibody - anti- Testosterone antibody - HRP-labeled Testosterone complex. The amount of bound labeled Testosterone is inversely proportional to that of Testosterone in the samples. The TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Testosterone in the samples is then determined by comparing the OD of the samples to the standard curve.



      Assay type

      Competitive

      Format

      96T

      Assay time

      2h

      Reactivity

      Universal

      Detection Method

      Colormetric

      Detection Range

      0.1—20 ng/mL

      Sensitivity

      0.1 ng/mL

      Sample Volume

      50μL

      Sample Type

      Serum, plasma and other biological fluids


      Specificity

      l No significant cross-reactivity or interference with progesterone, dihydrotestosterone, estradiol.      


      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received


      Item

      Specifications

      Storage

      Micro ELISA Plate(Dismountable)

      8 wells ×12 strips

      4

      Reference Standard (6 tubes)

      0.5mL /tube

      4

      Detection Ab

      1vial  6mL

      4

      HRP-labeled Testosterone

      1vial  6mL

      4(shading light)

      Concentrated Wash Buffer (20×)

      1vial  15mL

      4

      Substrate Reagent A

      1vial  7mL

      4(shading light)

      Substrate Reagent B

      1vial  7mL

      4(shading light)

      Stop Solution

      1vial  7mL

      4

      Plate Sealer

      5pieces

       

      Product Description

      1 copy

       

      Certificate of Analysis

      1 copy

       


      #: A set of Standard concentrations is 0 ng/mL, 0.1 ng/mL, 0.4 ng/mL, 1.6 ng/mL, 5 ng/mL, 20 ng/mL.

      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.



      Other supplies required

      Microplate reader with 450nm wavelength filter

      High-precision transferpettor, EP tubes and disposable pipette tips

      37 Incubator

      Deionized or distilled water

      Absorbent paper

      Loading slot for Wash Buffer


      Citations

      1. Publication: Li H, He Y, Zhang H, et al. Differential Proteome And Gene Expression Reveal Response To Carbon Ion Irradiation In Pubertal Mice Testes[J]. Toxicology Letters, 2014, 225(3): 433-444.
        Sample Type: Serum
      2. Publication: Yan W J, Mu Y, Yu N, et al. Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet[J]. Journal of Assisted Reproduction & Genetics, 2015, 32(7): 1097-1104.
        Sample Type: Serum
      3. Publication: Li J, Chang J, Li W, et al. Disruption Of Sex-Hormone Levels And Steroidogenic-Related Gene Expression On Mongolia Racerunner (Eremias Argus) After Exposure To Triadimefon And Its Enantiomers[J]. Chemosphere, 2017, 171: 554-563.
        Sample Type: Serum
      4. Publication: Yang M, Yan W J, Yin T L, et al. Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction[J]. Molecular Medicine Reports, 2016, 14(4): 3588-3594.
        Sample Type: Serum
      5. Publication: Cui X R, Long C L, Tian J, et al. Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR[J]. Cellular Physiology and Biochemistry, 2017, 41: 598-608.
        Sample Type: Serum
      6. Publication: Mu Y, Yan W J, Yin T L, et al. Curcumin Ameliorates High‑Fat Diet‑Induced Spermatogenesis Dysfunction[J]. Molecular Medicine Reports, 2016, 14(4): 3588-3594.
        Sample Type: Serum
      7. Publication: Lee H S, Lee Y J, Chung Y H, et al. In Vitro And In Vivo Evaluation Of Tissue-Cultured Mountain Ginseng On Penile Erection[J]. Journal of Ginseng Research, 2016, 40(4): 334-343.
        Sample Type: Serum
      8. Publication: Mu Y, Yan W, Yin T, et al. Diet-Induced Obesity Impairs Spermatogenesis: A Potential Role For Autophagy[J]. Scientific Reports, 2017.
        Sample Type: Serum
      9. Publication: Zheng Q, Li Y L, Zhang D D, et al. ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats[J]. Cell Death & Disease, 2017.
        Sample Type: Serum
      10. Publication: Kong L H, Wang Q E, Jin J W, et al. Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells[J]. Plos One, 2017.
        Sample Type: culture supernatant
      11. Publication: Ommati M M, Heidari R, Jamshidzadeh A, et al. Dual Effects Of Sulfasalazine On Rat Sperm Characteristics, Spermatogenesis, And Steroidogenesis In Two Experimental Models[J]. Toxicology Letters, 2017.
        Sample Type: Serum
      12. Publication: Erkan L G, Altinbas B, Guvenc G, et al. Brain Thromboxane A2 Via Arachidonic Acid Cascade Induces The Hypothalamic-Pituitary-Gonadal Axis Activation In Rats[J]. Autonomic Neuroscience-Basic & Clinical, 2015, 189: 50-55.
        Sample Type: Plasma
      13. Publication: Amidu N, Alidu H, Owiredu W, et al. Adequate Exercise Improves Inflammatory, Gonadal Markers and Sexual Function in Type 2 Diabetics[J].Journal of Advances in Medicine and Medical Research, 2018, 25(8): 1-8.
      14. Publication: Mu Y, Yin T, Yin L, et al. CTRP3 attenuates high-fat diet-induced male reproductive dysfunction in mice[J]. Clinical Science, 2018: CS20180179.
      15. Publication: Tang N, Liu L, Qiu H, et al. Analysis of gene expression and functional changes of adrenal gland in a rat model of kidney yang deficiency syndrome treated with Sini decoction[J]. Experimental and Therapeutic Medicine.
        Sample Type: Serum
      16. Publication: Orji O C, Uchendu I K, Agu C E, et al. Short Communication Combined Effects of Vitamin C and Tomato Extract (Lycopersicon Esculentum) on Carbimazole-induced Alterations in the Testes of Male Albino Rats[J]. Indian J Physiol Pharmacol, 2018, 62(3): 380-384.
        Sample Type: Serum
      17. Publication: Tang X, Wu S, Shen L, et al. Di‐(2‐ethylhexyl) phthalate (DEHP)‐induced testicular toxicity through Nrf2‐mediated Notch1 signaling pathway in Sprague–Dawley rats[J]. Environmental toxicology, 2018.
        Sample Type: rats
      18. Publication: Wang, M., Wang, Y., Xie, T. et al. Prostaglandin E2/EP2 receptor signalling pathway promotes diabetic retinopathy in a rat model of diabetes[J]. Diabetologia (2018).
        Sample Type: Human
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