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MAGEC2 Polyclonal Antibody

Cat:E-AB-19007
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:293T,Jurkat
Verified Samples in IHC:Human cervical cancer,Human colorectal cancer
Dilution

WB 1:1000-1:5000, IHC 1:50-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Fusion protein of human MAGEC2
Abbre MAGEC2
Synonyms Cancer testis antigen 10;Cancer/testis antigen 10;CT10;HCA587;Hepatocellular cancer antigen 587;Hepatocellular carcinoma associated antigen 587;Hepatocellular carcinoma-associated antigen 587;MAGC2;MAGE C2 antigen;MAGE E1 antigen;MAGE family member C2;MAGE-C2 antigen;MAGE-E1 antigen;MAGEC2;Melanoma antigen family C 2;Melanoma antigen family C2;Melanoma antigen family E 1 cancer/testis specific;Melanoma associated antigen C2;Melanoma-associated antigen C2;MGC13377
Swissprot
Calculated MW 41 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Nucleus. Nuclear in germ cells. Cytoplasmic in well-differentiated hepatocellular carcinoma, nuclear in moderately- and poorly-differentiated hepatocellular carcinoma.
Concentration 1.08 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cancer; Immunology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene is a member of the MAGEC gene family. It is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and the other MAGEC genes are clustered on chromosome Xq26-q27.