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BSG Polyclonal Antibody

Cat:E-AB-40297
Manual MSDS
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Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Mouse;Rat
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Rat liver,Mouse kidney,Rat heart,Mouse liver
Verified Samples in IHC:Mouse liver
Dilution

WB 1:1000-1:2000 , IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant Mouse Basigin protein
Abbre BSG
Synonyms 5A11 antigen;5F7;BASI;Basigin (Ok blood group);Basigin;Blood brain barrier HT7 antigen;Bsg;CD 147;CD147;CD147 antigen;Collagenase stimulatory factor;EMMPRIN;Extracellular matrix metalloproteinase inducer;Leukocyte activation antigen M6;M 6;M6;M6 leukocyte activation antigen;Neurothelin;OK;OK blood group;OK blood group antigen;TCSF;Tumor cell derived collagenase stimulatory factor;Tumor cell-derived collagenase stimulatory factor
Swissprot
Calculated MW 30kDa,40-60kDa
Observed MW 30kDa,40-60kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane
Concentration 1 mg/mL
Buffer PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Purification Method Antigen Affinity Purification
Research Areas Cancer; Cardiovascular; Metabolism; Immunology; Neuroscience
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background BSG,also named as 5F7,Basigin,EMMPRIN,TCSF and CD147,plays pivotal roles in spermatogenesis,embryo implantation,neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1,SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA,inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix,plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.