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LYZ Polyclonal Antibody

Cat:E-AB-64686
Manual MSDS
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Price: $ 530

Price: $ 320

Price: $ 200

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:HL-60,U937,Mouse lung
Verified Samples in IHC:Human liver cancer,Human stomach,Mouse lung,Mouse kidney
Dilution

WB 1:500-1:2000, IHC 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen A synthetic peptide of human LYZ (NP_000230.1).
Abbre LYZ
Synonyms LYZ;LYZF1;LZM;lysozyme
Swissprot
Calculated MW 16kDa
Observed MW 13kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm
Concentration 1mg/mL
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Cancer; Cardiovascular; Immunology; Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.