PSME3 Polyclonal Antibody
- +1
Price: $ 530
Price: $ 320
Price: $ 200
Size:
200μL
120μL
60μL
Quantity:
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: IF
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Product Details
Verified Samples |
Verified Samples in IF: C6, NIH/3T3, U-2OS |
Dilution |
IF 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | A synthetic peptide of human PSME3 (NP_005780.2). |
Abbre | PSME3 |
Synonyms | PSME3;HEL-S-283;Ki;PA28-gamma;PA28G;PA28gamma;REG-GAMMA |
Swissprot | |
Cellular Localization | Nucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Immunology |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. |
Experiment Operation Video
Protocols
• Western Blot Guide
• Immunohistochemistry Guide for Slide-mounted Paraffin Sections
• Immunofluorescence Guide
• Immunohistochemistry Troubleshooting Tips
• Western Blot Troubleshooting Tips
• Immunofluorescence Troubleshooting Tips
• Comparison of Protein Separation Linear Range
• ImmunoPrecipitation Protocol